INHIBITORY EFFECT OF THIOPHOSPHORIC ACID ALKALOID DERIVATIVES FROM CHELIDONIUM MAJUS L. (UKRAIN) ON OVALBUMIN ANTIGENICITY AND ANTIOVALBUMIN IgE ANTIBODY RESPONSE IN MICE
WYCZÓŁKOWSKA J.1*, MICHOŃ T.1, NOWICKY J.W.2
1) Department of Biogenic Amines, Polish Academy of Sciences,
2) Ukrainian Anti-Cancer Institute, 1040 Vienna, Austria.
* Address for correspondence: Janina Wyczółkowska, Department of Biogenic Amines, Polish Academy of Sciences, Tylna 3, 90-950 Łódź, P.O. Box 225, Poland.
Summary: The ability of the Chelidonium majus L alkaloid derivative Ukrain (UK) to inhibit ovalbumininduced sensitization was tested in BALB/c and F1(BALB/c x C57BL/6J) mice. UK introduced into the mice in the mixture with antigen (ovalbumin) and adjuvant (alum) inhibited the sensitization of mice, reflected in lower anti-OA IgE antibody response and decreased antigen-induced histamine release from mast cells isolated from peritoneal cavities of sensitized mice. The effect of UK on the antigenicity of ovalbumin (OA) in anaphylaxis was tested in heterologous passive cutaneous anaphylactic (PCA) reaction on rats. The results show that the OA prepared in the mixture with UK had a decreased ability to react with anti-OA IgE antibodies raised against native OA in mice and fixed on the surface of rat mast cells in heterologous PCA reactions. The results suggest that UK pretreatment of OA may affect its anti-genie property and the ability to react with anti-OA IgE antibodies raised against the native IgE molecules.